Cell Biology Labs 2008/2009

MAKE UP FOR MISSED LABS!

Those students, who were sent away from a lab practice because of inappropriate preparation or have missed one or more for any other reason, are expected to make up for their missed practices on week 10 and 11 (no other times are possible).
The procedure for doing spare lab (one or more) is the following.
1) According to the lab schedule (see below TIMETABLE FOR PRACTICES) you have to choose a fitting date in week 10 and/or week 11 (Week 10: 14-17 of April, Monday, 13 of April is Holliday!; week 11: 20-24 of April),
2) Next, you have to register, by sending an email to nizsaloe@dote.hu (Enikő Nizsalóczki, room #402 in the TB) indicating
3) which day (date), and
4) for what practice (title of practice) you sign up. She will respond to your registration.
5) At the negotiated time of the practices, you make up your missed practices.
6) Since you will get your final signature from your regular lab teachers, let them know when you will have accomplished all your four labs.

The laboratory practices begin on the second week!
Location of laboratory practices is the TB (biophysics laboratory rooms).

All university students have to prepare for the practices. It is not possible to do the practice in the case of lack of preparation. Making up for a missed practice with a written permit on the last practice is possible.

Cell biology practices are carried out in subgroups within the groups. The practices are made in 4 subgroups (A-D).

TIMETABLE FOR PRACTICES:

Abbreviations

PRACTICES:

Material to be studied for the lab is in "Cell Biology Lab Manual" 2003 edition, available in the University book store <<Medicina>>

LABORATORY WORKING SCHEDULE:

Image galery for labs (images open in new window):

electron microscopic morphology of reversible and irreversible cell damage
Nuclei stained with PI
EGF receptors on A431 epidermoid carcinoma cells
Mitosis of A431 cells - EGFR stained red (Cy3-antiEGFR-antibody), actin-cytoskeleton green (FITC-phalloidine), nuclei blue (DAPI)
Fluorescence in situ hybridisation (FISH)
structure of chromatin
model of nuclear halo
comet essay - image analysis
DNA damage analysed by comet essay
apoptosis: comet and halo essays

LABORATORY SAFETY INSTRUCTIONS:

The lab is a dangerous working place, therefore every student should work with caution, paying attention especially to the following things:

1. Eating, drinking and smoking are forbidden in the lab.
2. You are required to work with caution with acids, bases and other toxic chemicals (these will be labeled). Extra care has to be taken when working with the following chemicals:
methanol (might cause blindness), azide and concentrated hydrochloric acid, ethidium bromide, propidium idodie (carcinogenic), phalloidin (toxin of death-cap).
3. In case of injury or spillage of dangerous or potentially infectious material call the practice leader immediately.
4. Work very cautiously with potentially infectious materials (blood and cultured cells). Although the blood testing procedure (for infectious agents) complies with the requirements of the National Hematology and Blood Transfusion Institution, you are required to wear gloves when handling blood and any instrument that may contain contamination of potentially infectious material.
5. Manipulating with gas taps is forbidden.
6. Touching the 220 V and 380 V electric sources is extremely dangerous.
7. It is not allowed to turn the fluorescent microscopes on and off repeatedly. Fluorescent microscopes contain a high pressure light source. Never take the housing of the bulb apart. The surface of the bulb housing may be hot.
8. Centrifuges can be switched on only in the presence of the practice leader.
9. In case of instrumental error or breaking, call the practice leader. Do not try to repair any device on your own.
10. Dispose of used material properly. Pay extra attention to potentially infectious or toxic material or glass slides. Ask your practice leader for instructions.
11. Clean up your work bench after finishing your practice. Clean the objectives of microscopes of immersion oil with isopropanol.
12. Completing all practicals, and writing up the results (including drawings) and their interpretation in a lab log on the spot is required. The lab tutor signs, on the cover of the log book, both for the presence at and the acceptance of the lab at the end of the practical. Those who do not have all their labs accepted are not entitled for the end of term signature in their lecture book.
13. Upon missing a lab, only in the case of serious illness or other similar event is extra lab tuition offered. This has to be confirmed by the study advisor who writes the permisson into the lab log. Please see the study advisor in office hour if you have such a problem - bring along your certificate of absence and your lab log book. This is a general permission and the student can only do the lab with another group if the other group's tutor agrees.